willaertia magna c2c maky  (ATCC)

Journal: Pathogens

Article Title: Mitigation of Expression of Virulence Genes in Legionella pneumophila Internalized in the Free-Living Amoeba Willaertia magna C2c Maky

doi: 10.3390/pathogens9060447

Figure Lengend Snippet: Level of rpsL transcript in the different conditions, expressed as the number of copies in 1 × 10 6 L. pneumophila (Lp) ± SD and displayed using a log scale. Abbreviations: FREE: L. pneumophila strains alone; WILL: L. pneumophila strains cocultured with W. magna C2c Maky; ACANTH: L. pneumophila strains cocultured with A. castellanii ; T′0: reference transcript level; 3D: transcript level after 3 days. ANOVA 2: Factor 1: “ L. pneumophila strain”, p < 0.0001; Factor 2: “culture conditioned”, p < 0.0001; Interaction Factor 1 × Factor 2, p < 0.0001.

Article Snippet: The amoebae used in this study were W. magna C2c Maky ATCC PTA-7824 and A. castellanii ATCC 30010.

Techniques:

Journal: Pathogens

Article Title: Mitigation of Expression of Virulence Genes in Legionella pneumophila Internalized in the Free-Living Amoeba Willaertia magna C2c Maky

doi: 10.3390/pathogens9060447

Figure Lengend Snippet: Measurement of the virulence index. ( A ) After 3 days in liquid medium; ( B ) after 3 days in liquid medium plus 3 days on buffered charcoal yeast extract (BCYE) plates. FREE: L. pneumophila strains alone; WILL: L. pneumophila strains cocultured with W. magna C2c Maky; ACANTH: L. pneumophila strains cocultured with A. castellanii ; T′0: reference virulence index; T3D: virulence index after 3 days; T6D: virulence index after 6 days, 3 days in liquid medium, and 3 days on BCYE plates.

Article Snippet: The amoebae used in this study were W. magna C2c Maky ATCC PTA-7824 and A. castellanii ATCC 30010.

Techniques:

Journal: Scientific Reports

Article Title: Investigation of potential pathogenicity of Willaertia magna by investigating the transfer of bacteria pathogenicity genes into its genome

doi: 10.1038/s41598-019-54580-6

Figure Lengend Snippet: Summary of the W. magna c2c genome.

Article Snippet: W. magna c2c maky cells (ATCC PTA-7824) were grown at 30 °C in 175 cm2 culture flasks (Thermo Fisher Scientific, Illkirch, France) containing 75 mL of SCGYEM medium .

Techniques: Sequencing

Journal: Scientific Reports

Article Title: Investigation of potential pathogenicity of Willaertia magna by investigating the transfer of bacteria pathogenicity genes into its genome

doi: 10.1038/s41598-019-54580-6

Figure Lengend Snippet: Comparison of the main genomic features of several amoebas.

Article Snippet: W. magna c2c maky cells (ATCC PTA-7824) were grown at 30 °C in 175 cm2 culture flasks (Thermo Fisher Scientific, Illkirch, France) containing 75 mL of SCGYEM medium .

Techniques: Comparison

Journal: Scientific Reports

Article Title: Investigation of potential pathogenicity of Willaertia magna by investigating the transfer of bacteria pathogenicity genes into its genome

doi: 10.1038/s41598-019-54580-6

Figure Lengend Snippet: Representation of horizontal transfer analysis. Phylogenetic trees for two W. magna c2c maky proteins of putative ARB origin. The trees were constructed using maximum-likelihood method based on the leucine rich repeat sequence of W. magna . The trees were performed with 18 ( a ) and 14 ( b ) homologous sequences of W. magna retrieved by BLASTp on NCBI. In red: W. magna c2c maky leucine rich repeat-containing protein ( a ) gene 14,561; ( b ) gene 11,963); in green: the ARB ( legionella geestiana ) homologs; in blue: homologs from legionella geestiana ( a ) or homologs from giant viruses ( b ); in black: homologs from other organisms.

Article Snippet: W. magna c2c maky cells (ATCC PTA-7824) were grown at 30 °C in 175 cm2 culture flasks (Thermo Fisher Scientific, Illkirch, France) containing 75 mL of SCGYEM medium .

Techniques: Construct, Sequencing

Journal: Scientific Reports

Article Title: Investigation of potential pathogenicity of Willaertia magna by investigating the transfer of bacteria pathogenicity genes into its genome

doi: 10.1038/s41598-019-54580-6

Figure Lengend Snippet: Phylogenetic analysis of W. magna c2c maky Nf314 protein. The phylogenetic tree is based on protein Nf314 from W. magna c2c and other eukaryotic organisms including amoebas. Nf314 protein sequences of W. magna are not clustered with this homolog in N. fowleri . The protein Nf314 of W. magna c2c is indicated in green; that of non-pathogenic amoeba in blue and that of pathogenic amoeba ( N. fowleri ) in red.

Article Snippet: W. magna c2c maky cells (ATCC PTA-7824) were grown at 30 °C in 175 cm2 culture flasks (Thermo Fisher Scientific, Illkirch, France) containing 75 mL of SCGYEM medium .

Techniques:

Journal: FEMS microbiology letters

Article Title: Free-living freshwater amoebae differ in their susceptibility to the pathogenic bacterium Legionella pneumophila.

doi: 10.1111/j.1574-6968.2008.01387.x

Figure Lengend Snippet: Fig. 1. Effect of Legionella pneumophila on the growth of different amoebae in coculture. (a) Effect of the bacterial infection on the growth of amoebae. The different amoebae were cultured at 37 1C for up to 4 days (d3 and d4 represent, respectively, day 3 and day 4 in culture) either with (grey bars) or without (black bars) L. pneumophila Paris at an MOI of 50. White bars represent the number of amoebae on day 0 of experiments. The data are expressed as the number of amoebae mL1 of medium and are the mean SEM of four to eight independent experiments. Statistical differences (Student’s t-test) between the growth of amoebae cultured either with or without bacteria are indicated (P o 0.01; P o 0.001). (b and c) Effect of L. pneumophila on monolayer formation by amoebae. Representative phase-contrast images of the different amoebae cultured in 24-well plates either with or without the strains of L. pneumophila at an MOI of 50 for 3 days (b) or 7 days (c). Note the destruction of monolayers of Hartmannella and Acanthamoeba cultured in the presence of the bacteria when compared with controls and also the relative resistance of Willaertia magna (strain c2c). Also, note the destruction of monolayers of W. magna Z503 cultured in the presence of the bacteria for 7 days when compared with controls and the c2c strain.

Article Snippet: The amoebae used in this study were A. castellanii (By 02.2.4) and H. vermiformis (Ax.5.2e4b), two species known to support L. pneumophila and two strains of Willaertia genus, hitherto untested: W. magna, Z 503 (ATCC 50035) and c2c Maky (ATCC PTA-7824).

Techniques: Infection, Cell Culture, Bacteria

Journal: FEMS microbiology letters

Article Title: Free-living freshwater amoebae differ in their susceptibility to the pathogenic bacterium Legionella pneumophila.

doi: 10.1111/j.1574-6968.2008.01387.x

Figure Lengend Snippet: Fig. 3. Ultrastructure of Acanthamoeba castellanii and Willaertia magna infected by Legionella pneumophila after 36 h in coculture. (a, d and g) Different stages of A. castellanii infection by the L. pneumophila Paris. Micrographs (b), (e) and (h) show the few cells (see Table 1 for details) of W. magna c2c that were found to be infected by L. pneumophila Paris. (c, f and i) Typical W. magna Z503 infected by L. pneumophila Lens. (a) First stage of A. castellanii infection: the bacteria are contained in phagosomes surrounded by a rough endoplasmic reticulum (rer). (b) Single L. pneumophila not surrounded by rer in W. magna c2c. (c) Willaertia magna Z503 displaying a clearly delimited replicative phagosome filled with L. pneumophila Lens. (d) Massively infected prelytic A. castellanii displaying a contractile vacuole (CV). N, nucleus. (e) One of the two W. magna c2c (2/133 cells observed) displaying a replicative phagosome. (f) Massively infected prelytic W. magna Z503. (g–i) Different morphological aspects of L. pneumophila Paris internalized within Acanthamoeba (g) and Willaertia c2c (h) and of L. pneumophila Lens within Willaertia Z503 (i). Bacteria are indicated by arrows on (b), (c), (f), (g), (h) and (i).

Article Snippet: The amoebae used in this study were A. castellanii (By 02.2.4) and H. vermiformis (Ax.5.2e4b), two species known to support L. pneumophila and two strains of Willaertia genus, hitherto untested: W. magna, Z 503 (ATCC 50035) and c2c Maky (ATCC PTA-7824).

Techniques: Infection, Bacteria